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complex v activity assay kit  (Elabscience Biotechnology)


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    Elabscience Biotechnology complex v activity assay kit
    293 T cells were transfected with empty vector, wild-type Cend1, or the Cend1-G130P mutant for 48 h, followed by oxygen–glucose deprivation/reoxygenation (OGD/R) treatment. Cells were collected 24 h after reoxygenation for subsequent analyses. A Representative flow cytometric analysis of JC-1 staining showing the proportion of monomers and aggregates. B Quantification of JC-1 monomer proportion. N = 3. C Representative flow cytometric analysis of calcein–cobalt staining to assess mPTP opening. D Quantification of calcein fluorescence intensity. N = 3. E Relative NADH–CoQ reductase (complex I) activity measured using a mitochondrial complex I assay kit. N = 3. F Relative F₁F₀-ATP synthase (complex V) activity measured using a mitochondrial <t>complex</t> <t>V</t> assay kit. N = 3. G Intracellular ATP levels measured by ATP assay kit and normalized to protein content. N = 3. Data are presented as mean ± SEM. * P < 0.05; ** P < 0.01; ns, not significant.
    Complex V Activity Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complex v activity assay kit/product/Elabscience Biotechnology
    Average 93 stars, based on 8 article reviews
    complex v activity assay kit - by Bioz Stars, 2026-05
    93/100 stars

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    1) Product Images from "Targeting Cend1-Atp5f1b interaction rescues mitochondrial dysfunction and ameliorates ischemic brain injury"

    Article Title: Targeting Cend1-Atp5f1b interaction rescues mitochondrial dysfunction and ameliorates ischemic brain injury

    Journal: Communications Biology

    doi: 10.1038/s42003-025-09419-4

    293 T cells were transfected with empty vector, wild-type Cend1, or the Cend1-G130P mutant for 48 h, followed by oxygen–glucose deprivation/reoxygenation (OGD/R) treatment. Cells were collected 24 h after reoxygenation for subsequent analyses. A Representative flow cytometric analysis of JC-1 staining showing the proportion of monomers and aggregates. B Quantification of JC-1 monomer proportion. N = 3. C Representative flow cytometric analysis of calcein–cobalt staining to assess mPTP opening. D Quantification of calcein fluorescence intensity. N = 3. E Relative NADH–CoQ reductase (complex I) activity measured using a mitochondrial complex I assay kit. N = 3. F Relative F₁F₀-ATP synthase (complex V) activity measured using a mitochondrial complex V assay kit. N = 3. G Intracellular ATP levels measured by ATP assay kit and normalized to protein content. N = 3. Data are presented as mean ± SEM. * P < 0.05; ** P < 0.01; ns, not significant.
    Figure Legend Snippet: 293 T cells were transfected with empty vector, wild-type Cend1, or the Cend1-G130P mutant for 48 h, followed by oxygen–glucose deprivation/reoxygenation (OGD/R) treatment. Cells were collected 24 h after reoxygenation for subsequent analyses. A Representative flow cytometric analysis of JC-1 staining showing the proportion of monomers and aggregates. B Quantification of JC-1 monomer proportion. N = 3. C Representative flow cytometric analysis of calcein–cobalt staining to assess mPTP opening. D Quantification of calcein fluorescence intensity. N = 3. E Relative NADH–CoQ reductase (complex I) activity measured using a mitochondrial complex I assay kit. N = 3. F Relative F₁F₀-ATP synthase (complex V) activity measured using a mitochondrial complex V assay kit. N = 3. G Intracellular ATP levels measured by ATP assay kit and normalized to protein content. N = 3. Data are presented as mean ± SEM. * P < 0.05; ** P < 0.01; ns, not significant.

    Techniques Used: Transfection, Plasmid Preparation, Mutagenesis, Staining, Fluorescence, Activity Assay, ATP Assay

    Cells were collected 24 h after reperfusion for subsequent assays. A Cells were stained with JC-1 at working concentration, and the ratio of JC-1 monomers to aggregates was analyzed by flow cytometry. B Quantification of JC-1 monomer proportion. N = 3. C Cells were stained with MPTP detection reagent at working concentration, and green fluorescence intensity was analyzed by flow cytometry. D Quantification of green fluorescence intensity reflecting mPTP opening. N = 3. E NADH–CoQ reductase activity was measured using a mitochondrial complex I activity assay kit according to the manufacturer’s instructions, and relative activity was quantified. N = 3. F F₁F₀-ATP synthase activity was measured using a complex V activity assay kit and quantified as relative activity. N = 3. G Intracellular ATP content was measured using a luminescence-based ATP assay kit and quantified relative to control. N = 3. Data are presented as mean ± SEM. * P < 0.05; ** P < 0.01; ns, not significant.
    Figure Legend Snippet: Cells were collected 24 h after reperfusion for subsequent assays. A Cells were stained with JC-1 at working concentration, and the ratio of JC-1 monomers to aggregates was analyzed by flow cytometry. B Quantification of JC-1 monomer proportion. N = 3. C Cells were stained with MPTP detection reagent at working concentration, and green fluorescence intensity was analyzed by flow cytometry. D Quantification of green fluorescence intensity reflecting mPTP opening. N = 3. E NADH–CoQ reductase activity was measured using a mitochondrial complex I activity assay kit according to the manufacturer’s instructions, and relative activity was quantified. N = 3. F F₁F₀-ATP synthase activity was measured using a complex V activity assay kit and quantified as relative activity. N = 3. G Intracellular ATP content was measured using a luminescence-based ATP assay kit and quantified relative to control. N = 3. Data are presented as mean ± SEM. * P < 0.05; ** P < 0.01; ns, not significant.

    Techniques Used: Staining, Concentration Assay, Flow Cytometry, Fluorescence, Activity Assay, ATP Assay, Control



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    293 T cells were transfected with empty vector, wild-type Cend1, or the Cend1-G130P mutant for 48 h, followed by oxygen–glucose deprivation/reoxygenation (OGD/R) treatment. Cells were collected 24 h after reoxygenation for subsequent analyses. A Representative flow cytometric analysis of JC-1 staining showing the proportion of monomers and aggregates. B Quantification of JC-1 monomer proportion. N = 3. C Representative flow cytometric analysis of calcein–cobalt staining to assess mPTP opening. D Quantification of calcein fluorescence intensity. N = 3. E Relative NADH–CoQ reductase (complex I) activity measured using a mitochondrial complex I assay kit. N = 3. F Relative F₁F₀-ATP synthase (complex V) activity measured using a mitochondrial <t>complex</t> <t>V</t> assay kit. N = 3. G Intracellular ATP levels measured by ATP assay kit and normalized to protein content. N = 3. Data are presented as mean ± SEM. * P < 0.05; ** P < 0.01; ns, not significant.
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    293 T cells were transfected with empty vector, wild-type Cend1, or the Cend1-G130P mutant for 48 h, followed by oxygen–glucose deprivation/reoxygenation (OGD/R) treatment. Cells were collected 24 h after reoxygenation for subsequent analyses. A Representative flow cytometric analysis of JC-1 staining showing the proportion of monomers and aggregates. B Quantification of JC-1 monomer proportion. N = 3. C Representative flow cytometric analysis of calcein–cobalt staining to assess mPTP opening. D Quantification of calcein fluorescence intensity. N = 3. E Relative NADH–CoQ reductase (complex I) activity measured using a mitochondrial complex I assay kit. N = 3. F Relative F₁F₀-ATP synthase (complex V) activity measured using a mitochondrial complex V assay kit. N = 3. G Intracellular ATP levels measured by ATP assay kit and normalized to protein content. N = 3. Data are presented as mean ± SEM. * P < 0.05; ** P < 0.01; ns, not significant.

    Journal: Communications Biology

    Article Title: Targeting Cend1-Atp5f1b interaction rescues mitochondrial dysfunction and ameliorates ischemic brain injury

    doi: 10.1038/s42003-025-09419-4

    Figure Lengend Snippet: 293 T cells were transfected with empty vector, wild-type Cend1, or the Cend1-G130P mutant for 48 h, followed by oxygen–glucose deprivation/reoxygenation (OGD/R) treatment. Cells were collected 24 h after reoxygenation for subsequent analyses. A Representative flow cytometric analysis of JC-1 staining showing the proportion of monomers and aggregates. B Quantification of JC-1 monomer proportion. N = 3. C Representative flow cytometric analysis of calcein–cobalt staining to assess mPTP opening. D Quantification of calcein fluorescence intensity. N = 3. E Relative NADH–CoQ reductase (complex I) activity measured using a mitochondrial complex I assay kit. N = 3. F Relative F₁F₀-ATP synthase (complex V) activity measured using a mitochondrial complex V assay kit. N = 3. G Intracellular ATP levels measured by ATP assay kit and normalized to protein content. N = 3. Data are presented as mean ± SEM. * P < 0.05; ** P < 0.01; ns, not significant.

    Article Snippet: Activities of mitochondrial complex I and V in 293 T cells were measured using the Cell Mitochondrial Complex I Activity Assay Kit (Elabscience, #E-BC-K834-M) and Complex V Activity Assay Kit (Elabscience, #E-BC-K838-M), respectively, according to the manufacturer’s instructions.

    Techniques: Transfection, Plasmid Preparation, Mutagenesis, Staining, Fluorescence, Activity Assay, ATP Assay

    Cells were collected 24 h after reperfusion for subsequent assays. A Cells were stained with JC-1 at working concentration, and the ratio of JC-1 monomers to aggregates was analyzed by flow cytometry. B Quantification of JC-1 monomer proportion. N = 3. C Cells were stained with MPTP detection reagent at working concentration, and green fluorescence intensity was analyzed by flow cytometry. D Quantification of green fluorescence intensity reflecting mPTP opening. N = 3. E NADH–CoQ reductase activity was measured using a mitochondrial complex I activity assay kit according to the manufacturer’s instructions, and relative activity was quantified. N = 3. F F₁F₀-ATP synthase activity was measured using a complex V activity assay kit and quantified as relative activity. N = 3. G Intracellular ATP content was measured using a luminescence-based ATP assay kit and quantified relative to control. N = 3. Data are presented as mean ± SEM. * P < 0.05; ** P < 0.01; ns, not significant.

    Journal: Communications Biology

    Article Title: Targeting Cend1-Atp5f1b interaction rescues mitochondrial dysfunction and ameliorates ischemic brain injury

    doi: 10.1038/s42003-025-09419-4

    Figure Lengend Snippet: Cells were collected 24 h after reperfusion for subsequent assays. A Cells were stained with JC-1 at working concentration, and the ratio of JC-1 monomers to aggregates was analyzed by flow cytometry. B Quantification of JC-1 monomer proportion. N = 3. C Cells were stained with MPTP detection reagent at working concentration, and green fluorescence intensity was analyzed by flow cytometry. D Quantification of green fluorescence intensity reflecting mPTP opening. N = 3. E NADH–CoQ reductase activity was measured using a mitochondrial complex I activity assay kit according to the manufacturer’s instructions, and relative activity was quantified. N = 3. F F₁F₀-ATP synthase activity was measured using a complex V activity assay kit and quantified as relative activity. N = 3. G Intracellular ATP content was measured using a luminescence-based ATP assay kit and quantified relative to control. N = 3. Data are presented as mean ± SEM. * P < 0.05; ** P < 0.01; ns, not significant.

    Article Snippet: Activities of mitochondrial complex I and V in 293 T cells were measured using the Cell Mitochondrial Complex I Activity Assay Kit (Elabscience, #E-BC-K834-M) and Complex V Activity Assay Kit (Elabscience, #E-BC-K838-M), respectively, according to the manufacturer’s instructions.

    Techniques: Staining, Concentration Assay, Flow Cytometry, Fluorescence, Activity Assay, ATP Assay, Control